1HK4

HUMAN SERUM ALBUMIN COMPLEXED WITH THYROXINE (3,3',5,5'-TETRAIODO-L-THYRONINE) and myristic acid (tetradecanoic acid)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	298
Detector technology	CCD
Collection date	1999-01-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	189.580, 38.910, 95.260
Unit cell angles	90.00, 105.43, 90.00

Refinement procedure

Resolution	37.500 * - 2.400
R-factor	0.216
Rwork	0.216
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e7g
RMSD bond length	0.007 *
RMSD bond angle	1.180 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.221
Number of reflections	24201
<I/σ(I)>	5.6	2.7
Completeness [%]	90.6	76
Redundancy	2.1	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	100 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)	pH7.0-7.5