1HK0

Human GammaD Crystallin Structure at 1.25 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.220, 52.971, 90.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.170 - 1.250
R-factor	0.1671 *
Rwork	0.164
R-free	0.20780 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1elp
RMSD bond length	0.786 *
RMSD bond angle	1.529 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.170	1.320
High resolution limit [Å]	1.250	1.250
Rmerge	0.048	0.189
Total number of observations	212732 *
Number of reflections	39021 *
<I/σ(I)>	7.4	3.7
Completeness [%]	86.8	45.5
Redundancy	2.9	1.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	277	10MM PHOSPHATE, 4 DEGREE CELSIUS, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium phosphate	100 (mM)	pH7.
2	1	1	dithiothreitol	20 (ml)