1HJZ
Crystal structure of AF1521 protein containing a macroH2A domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.930, 55.720, 62.740 |
Unit cell angles | 90.00, 115.15, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.185 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.100 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.047 * | 0.151 |
Number of reflections | 40692 | |
<I/σ(I)> | 19.1 | 6.7 |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 3.4 * | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | 17 * | 0.1M MES BUFFER, PH 6.5 18% PEG 5000 MME, 0.2M AMMONIUM SULPHATE, 25MM MANGANESE CHLORIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG5000 MME | 18 (%(w/v)) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | 25 (mM) | ||
5 | 1 | drop | protein | 10 (mg/ml) | |
6 | 1 | drop | dithiothreitol | 5 (mM) |