1HJS

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Collection date	2001-11-15
Spacegroup name	C 1 2 1
Unit cell lengths	108.375, 136.145, 111.007
Unit cell angles	90.00, 119.02, 90.00

Refinement procedure

Resolution	20.000 * - 1.880 *
R-factor	0.194
Rwork	0.194
R-free	0.20800
Structure solution method	OTHER
RMSD bond length	0.005
RMSD bond angle	23.000 *
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.076	0.417
Number of reflections	114360
Completeness [%]	97.6	89
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		30% PEG4000, 0.2 M AMMONIUM SULPHATE, 0.1M HEPES PH=7.5, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	HEPES	0.1 (M)	pH7.5