1HJP
HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-29 |
| Detector | RIGAKU |
| Spacegroup name | P 4 |
| Unit cell lengths | 83.730, 83.730, 34.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| Rwork | 0.229 |
| R-free | 0.29400 |
| Structure solution method | MIRAS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.850 |
| Number of reflections | 7922 | |
| <I/σ(I)> | 8.4 | 1.9 |
| Completeness [%] | 93.7 | 80.5 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 4.8 * | 20 * | 50MM CITRATE PH4.9 500MM NACL 10% GLYCEROL 3% PEG2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | citrate | 50 (mM) | |
| 2 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 3 | 1 | 1 | 500 (mM) | ||
| 4 | 1 | 1 | glycerol | 10 (%) | |
| 5 | 1 | 1 | PEG2000 | 3-3.5 (%) |
