1HJL
Biochemical and Structural Analysis of the Molybdenum Cofactor Biosynthesis protein MobA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-30 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.224, 41.752, 54.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.230 - 2.000 |
Rwork | 0.171 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e5k |
RMSD bond length | 0.020 |
RMSD bond angle | 1.851 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.163 |
Number of reflections | 12166 | |
<I/σ(I)> | 17.9 | 4.4 |
Completeness [%] | 98.1 | 86.9 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Stevenson, C.E., (2000) Structure, 8, 1115. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
3 | 1 | reservoir | PEG1500 | 2 (%(w/v)) | |
4 | 1 | reservoir | citric acid | 100 (mM) |