1HIZ

Xylanase T6 (Xt6) from Bacillus Stearothermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-15
Detector	MARRESEARCH
Wavelength(s)	0.88,0.9791,0.97
Spacegroup name	P 32 2 1
Unit cell lengths	112.870, 112.870, 122.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 2.400
R-factor	0.156 *
Rwork	0.158
R-free	0.18600 *
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.055 *	0.211 *
Total number of observations	161549 *
Number of reflections	35642 *
<I/σ(I)>	12.2	3
Completeness [%]	99.8 *	100 *
Redundancy	4.5 *	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		Teplitsky, A., (1997) Acta Cryst., D53, 608. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-6 (mg/ml)
2	1	drop	ammonium sulfate	0.7-1.0 (M)
3	1	reservoir	ammonium sulfate	1.4-2.0 (M)