1HIL
STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIGEN-ANTIBODY RECOGNITION
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.040, 82.800, 73.400 |
| Unit cell angles | 90.00, 122.60, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.090 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 10 (mg/ml) | |
| 2 | 1 | drop | imidazole malate | 0.2 (M) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | PEG600 | 30-39 (%) | |
| 5 | 1 | reservoir | imidazole malate | 0.2 (M) | |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | PEG600 | 30-39 (%) |
