1HIJ

INTERLEUKIN-4 MUTANT WITH ARG 88 REPLACED WITH GLN (R88Q)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-02-02
Detector	SIEMENS
Spacegroup name	P 41 21 2
Unit cell lengths	92.100, 92.100, 46.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.224
Rwork	0.224
R-free	0.31600
RMSD bond length	0.005
RMSD bond angle	2.290 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.168
Number of reflections	5814
Completeness [%]	91.0
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	60-65 (%sat)
3	1	reservoir	2-N-morpholino-ethane-sulfonate		or citrate