1HIB

THE STRUCTURE OF AN INTERLEUKIN-1 BETA MUTANT WITH REDUCED BIOACTIVITY SHOWS MULTIPLE SUBTLE CHANGES IN CONFORMATION THAT AFFECT PROTEIN-PROTEIN RECOGNITION

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	55.430, 55.430, 241.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.19
Rwork	0.190
RMSD bond length	0.017
RMSD bond angle	0.046 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.270 *
Rmerge	0.062 *
Total number of observations	30094 *
Number of reflections	8534 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.4 (mg/ml)
2	1	drop	ammonium sulfate	0.168 (M)
3	1	reservoir	ammonium sulfate	2.1 (M)