1HI8
RNA dependent RNA polymerase from dsRNA bacteriophage phi6
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-15 |
| Detector | SBC |
| Wavelength(s) | 0.97923, 0.97939, 0.99988, 0.92873 |
| Spacegroup name | P 32 |
| Unit cell lengths | 110.000, 110.000, 159.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.31600 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.240 |
| Total number of observations | 430000 * | |
| Number of reflections | 66000 | |
| <I/σ(I)> | 17.2 | 1.4 |
| Completeness [%] | 88.0 | 66 * |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.7 | 293 * | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, 56, 1473. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) |
