1HHT
RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus template
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.089, 92.239, 140.640 |
| Unit cell angles | 90.00, 101.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TBA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.170 | 0.960 |
| Number of reflections | 56000 | |
| <I/σ(I)> | 7.1 | 1.1 |
| Completeness [%] | 95.0 | 86 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.7 | 293 * | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, 56, 1473. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) |
