1HHS

RNA dependent RNA polymerase from dsRNA bacteriophage phi6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	105.408, 93.364, 141.199
Unit cell angles	90.00, 101.25, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.2212
Rwork	0.221
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TBA
RMSD bond length	0.016
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.440
Total number of observations	811000 *
Number of reflections	181000
<I/σ(I)>	21	2
Completeness [%]	100.0	100
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7	293 *	Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)	pH6.7