1HHP

THE THREE-DIMENSIONAL STRUCTURE OF THE ASPARTYL PROTEASE FROM THE HIV-1 ISOLATE BRU

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	50.200, 50.200, 106.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.700
R-factor	0.19 *
Rwork	0.190
RMSD bond length	0.018
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.098 *
Total number of observations	3901 *
Number of reflections	3549 *
Completeness [%]	93.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HIV-protase	1.1 (mg/ml)
2	1	reservior	MOPS	100 (mM)
3	1	reservoir		10 (%(w/v))
4	1	reservoir		0.4 (%(w/v))