1HH9

ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH A PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-15
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	102.720, 102.720, 295.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.000 - 2.700
R-factor	0.282
Rwork	0.282
R-free	0.33300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bog
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.084	0.451
Number of reflections	24567
<I/σ(I)>	14	2.7
Completeness [%]	95.6	98.4
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		HANGING DROP SET UP AT ROOM TEMPERATURE PROTEIN CONCENTRATION: 10 MG/ML RESERVOIR: 1.8 M AMMONIUM SULFATE, 0.1 M MOPS-BUFFER, PH 7.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8 (M)
3	1	reservoir	MOPS	0.1 (M)	pH7.5