1HH7
REFINED CRYSTAL STRUCTURE OF CYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS AT 1.4 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.670, 64.670, 68.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.000 - 1.400 |
| R-factor | 0.1508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.096 | 0.507 |
| Total number of observations | 394132 * | |
| Number of reflections | 31246 | |
| <I/σ(I)> | 5.9 | 5.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 12 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 291 * | 60% AMMONIUM SULPHATE, 0.1 M TRIS PH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.67 (mM) | 9.0mg/ml |
| 2 | 1 | drop | phosphate | 200 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 61 (%sat) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
