1HH5
cytochrome c7 from Desulfuromonas acetoxidans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.190, 43.830, 44.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.000 - 1.900 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1new |
RMSD bond length | 0.008 |
RMSD bond angle | 26.300 * |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 * | 0.182 * |
Number of reflections | 5213 | |
<I/σ(I)> | 5.6 | 2.1 |
Completeness [%] | 92.7 | 90 * |
Redundancy | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 | 20-22% PEG 1000, 0.1 M TRIS PH 7.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | |
3 | 1 | reservoir | PEG1000 | 20-22 (%) | |
4 | 1 | reservoir | Tris | 0.1 (M) |