1HH2

Crystal structure of NusA from Thermotoga maritima

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Wavelength(s)	0.950,0.98
Spacegroup name	P 43 21 2
Unit cell lengths	115.531, 115.531, 63.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.100
R-factor	0.2439
Rwork	0.244
R-free	0.31920
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.659
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.100
Rmerge	0.073 *	0.402 *
Number of reflections	25817 *
Completeness [%]	99.8 *	99.8 *
Redundancy	7.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	PEG400	2-5 (%)
3	1	reservoir	HEPES	100 (mM)