1HGX

HYPOXANTHINE-GUANINE-XANTHINE PHOSPHORIBOSYLTRANSFERASE (HGXPRTASE)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-08-16
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	48.960, 74.920, 55.720
Unit cell angles	90.00, 110.77, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.175
Rwork	0.175
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN HGPRTASE
RMSD bond length	0.008
RMSD bond angle	1.420
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.063	0.380 *
Total number of observations	97259 *
Number of reflections	27314
Completeness [%]	91.2	55.6 *
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *		22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	0.2 (M)
2	1	reservoir	Bis-Tris	0.1 (M)
3	1	reservoir	PEG8000	28-32 (%(w/v))
4	1	drop	HGX-PRTase	10 (mg/ml)
5	1	drop	Bis-Tris	50 (mM)
6	1	drop		6 (mM)