1HGW
CEL6A D175A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-29 |
| Detector | MSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.630, 74.720, 91.440 |
| Unit cell angles | 90.00, 103.64, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILDTYPE CEL6A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.118 |
| Number of reflections | 37025 | |
| <I/σ(I)> | 19.8 | 7.8 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | Bergfors, T., (1989) J.Mol.Biol, 209, 167. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | mPEG5000 | 20 (%) | |
| 2 | 1 | reservoir | MES | 20 (mM) | pH6.0 |
| 3 | 1 | reservoir | 10 (mM) |
