1HGU

HUMAN GROWTH HORMONE

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 41 21 2
Unit cell lengths	57.020, 57.020, 130.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.500
R-factor	0.212
Rwork	0.212
RMSD bond length	0.025
RMSD bond angle	2.600
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.075
Total number of observations	13055 *
Number of reflections	5364 *
Completeness [%]	66.6 *	37.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein dimer	30 (mg/ml)
2	1	drop	BES	0.1 (M)
3	1	reservoir	ethanol	25-28 (%)
4	1	reservoir	BES	0.1 (M)