1HG7

High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	32.218, 39.127, 44.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.150
R-factor	0.104 *
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gzi
RMSD bond length	0.015
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	SHELXL
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.108	0.493
Number of reflections	20883
<I/σ(I)>	5.2	3.7
Completeness [%]	99.9	100
Redundancy	6.7	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	20 *	50% AMMONIUM SULFATE, 0.1M SODIUM ACETATE, PH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	45 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)