1HG7
High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.218, 39.127, 44.694 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.150 |
R-factor | 0.104 * |
R-free | 0.16900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gzi |
RMSD bond length | 0.015 |
RMSD bond angle | 0.031 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | SHELXL |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.108 | 0.493 |
Number of reflections | 20883 | |
<I/σ(I)> | 5.2 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | 20 * | 50% AMMONIUM SULFATE, 0.1M SODIUM ACETATE, PH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 45 (%(w/v)) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) |