1HG2
CALM-N N-terminal domain of clathrin assembly lymphoid myeloid leukaemia protein, Inositol(4,5)P2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2000-04-27 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.859, 77.859, 122.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.940 - 2.000 |
R-factor | 0.19771 |
Rwork | 0.197 |
R-free | 0.21667 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hf8 |
RMSD bond length | 0.026 * |
RMSD bond angle | 2.200 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.126 | 1.958 |
Number of reflections | 25871 | |
<I/σ(I)> | 13.1 | 1.3 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 0.1M HEPES, PH 7.5, 12% PEG 8K, 8% ETHYLENE GLYCOL CRYSTALS SOAKED IN 1MM LIGAND FOR 1 HOUR |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | reservoir | PEG8000 | 12-14 (%) | |
3 | 1 | reservoir | ethylene glycol | 8 (%) |