1HG0

X-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and succinic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	MARRESEARCH
Wavelength(s)	1.5478
Spacegroup name	C 1 2 1
Unit cell lengths	107.180, 91.320, 128.410
Unit cell angles	90.00, 92.04, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.171
Rwork	0.171
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIUOSLY PUBLISHED STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINSE (MILLER ET AL. FEBS LETT. 1993
RMSD bond length	0.005
RMSD bond angle	23.620 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.109 *	0.384 *
Total number of observations	228741 *
Number of reflections	91478
<I/σ(I)>	8.3	2
Completeness [%]	92.4	75.8
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.4		Miller, M., (1993) FEBS Lett., 328, 275. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	50 (%)
2	1	reservoir	CHES	0.1 (M)
3	1	reservoir	PEG400	2 (%(w/v))