1HFZ
ALPHA-LACTALBUMIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87, 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.620, 60.130, 77.180 |
| Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.30300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.078 |
| Number of reflections | 18629 |
| Completeness [%] | 80.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 16 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.15 (M) | ||
| 3 | 1 | reservoir | PEG4000 | 20-25 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
