1HFU

TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS at 1.68 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.120, 84.560, 139.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.680
R-factor	0.181 *
Rwork	0.184
R-free	0.21900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a65
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.058	0.275
Number of reflections	61668
<I/σ(I)>	22.2	10
Completeness [%]	96.0	92 *
Redundancy	4.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291 *	Ducros, V., (1997) Acta Crystallogr.,Sect.D, D53, 605. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	reservoir	PEG8000	26-30 (%(v/v))
4	1	reservoir	acetate	0.1 (M)