1HFU
TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS at 1.68 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.120, 84.560, 139.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.680 |
R-factor | 0.181 * |
Rwork | 0.184 |
R-free | 0.21900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a65 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.058 | 0.275 |
Number of reflections | 61668 | |
<I/σ(I)> | 22.2 | 10 |
Completeness [%] | 96.0 | 92 * |
Redundancy | 4.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 * | Ducros, V., (1997) Acta Crystallogr.,Sect.D, D53, 605. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | acetate | 10 (mM) | |
3 | 1 | reservoir | PEG8000 | 26-30 (%(v/v)) | |
4 | 1 | reservoir | acetate | 0.1 (M) |