1HFA
CALM-N N-terminal domain of clathrin assembly lymphoid myeloid leukaemia protein, PI(4,5)P2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2000-03-23 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.725, 77.725, 122.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.940 - 2.000 |
R-factor | 0.19844 * |
Rwork | 0.197 |
R-free | 0.23012 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hf8 |
RMSD bond length | 0.032 * |
RMSD bond angle | 2.500 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.103 | 1.153 |
Number of reflections | 26075 | |
<I/σ(I)> | 14.3 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 0.1M HEPES, PH 7.5, 12% PEG 8K, 8% ETHYLENE GLYCOL, CRYSTALS SOAKED IN 1MM LIGAND FOR 1 HOUR |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | reservoir | PEG8000 | 12-14 (%) | |
3 | 1 | reservoir | ethylene glycol | 8 (%) |