1HEK
Crystal structure of equine infectious anaemia virus matrix antigen (EIAV MA)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9116, 0.9781, 0.9778 |
| Spacegroup name | P 65 |
| Unit cell lengths | 47.150, 47.150, 204.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.780 - 2.800 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.37300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.600 |
| Total number of observations | 19170 * | |
| Number of reflections | 6347 | 633 * |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 18 * | 30% PEG5000, 0.2M AMMONIUM SULFATE, 0.1M MES (PH6.5), 291K, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 4 | 1 | drop | protein | 2.5 (mg/ml) |
