1HDU
Crystal structure of bovine pancreatic carboxypeptidase A complexed with aminocarbonylphenylalanine at 1.75 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 297 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.570, 60.524, 74.410 |
| Unit cell angles | 90.00, 97.84, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.750 |
| R-factor | 0.187 * |
| Rwork | 0.198 |
| R-free | 0.21900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.235 * |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.750 | 1.900 * |
| Rmerge | 0.046 | 0.149 * |
| Number of reflections | 89359 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 90.4 | 77.4 * |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 0.02 (M) | pH7.5 |
| 3 | 1 | 1 | 1.2 (M) | ||
| 4 | 1 | 2 | 0.14 (M) | pH7.5 |
