1HDM

HISTOCOMPATIBILITY ANTIGEN HLA-DM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	95.390, 109.930, 105.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.500
R-factor	0.197
Rwork	0.197
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dlh
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.055 *	0.213 *
Number of reflections	18729	1837 *
<I/σ(I)>	27.7	6.9
Completeness [%]	96.2	96.3
Redundancy	7.3	7.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *	4 *	pH 5.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris	10 (mM)
3	1	2		1 (M)
4	1	2	ammonium sulfate	0.5 (M)
5	1	2	sodium citrate	0.1 (M)