1HDK
Charcot-Leyden Crystal Protein - pCMBS Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 49.547, 49.547, 261.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 29.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 * | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.127 |
| Total number of observations | 105494 * | |
| Number of reflections | 16447 * | |
| <I/σ(I)> | 12.7 | 3.4 |
| Completeness [%] | 87.7 * | 72.4 |
| Redundancy | 1.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 * | 16 * | Leonidas, D.D., (1995) Structure (London), 3, 1379. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris | 0.1 (M) | |
| 3 | 1 | reservoir | Tris-acetate | 0.2 (M) |
