1HDK

Charcot-Leyden Crystal Protein - pCMBS Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-02-15
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	49.547, 49.547, 261.645
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.199
Rwork	0.199
R-free	0.22100
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	29.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000 *	1.870
High resolution limit [Å]	1.800	1.800
Rmerge	0.079	0.127
Total number of observations	105494 *
Number of reflections	16447 *
<I/σ(I)>	12.7	3.4
Completeness [%]	87.7 *	72.4
Redundancy	1.5	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5 *	16 *	Leonidas, D.D., (1995) Structure (London), 3, 1379. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	reservoir	Tris-acetate	0.2 (M)