1HDF
Evolution of the eye lens beta-gamma-crystallin domain fold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Wavelength(s) | 0.95, 0.97885, 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.350, 41.350, 213.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.162 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.350 |
| Rmerge | 0.057 |
| Number of reflections | 10284 |
| <I/σ(I)> | 26.4 |
| Completeness [%] | 99.5 |
| Redundancy | 5.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | calcium chloride | 0.2 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | 2-propanol | 20 (%(w/v)) |
