1HDA

A NOVEL ALLOSTERIC MECHANISM IN HAEMOGLOBIN. STRUCTURE OF BOVINE DEOXYHAEMOGLOBIN, ABSENCE OF SPECIFIC CHLORIDE-BINDING SITES AND ORIGIN OF THE CHLORIDE-LINKED BOHR EFFECT IN BOVINE AND HUMAN HAEMOGLOBIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	63.820, 95.510, 105.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500 *
R-factor	0.18 *
R-free	0.24900 *
RMSD bond length	0.009
RMSD bond angle	0.032
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.068 *
Total number of observations	133756 *
Number of reflections	32583 *
Completeness [%]	97.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG1000	50 (%(w/w))
2	1	reservoir	sodium phosphate	50 (mM)	can be replaced with 0.1M HEPES