1HD2
Human peroxiredoxin 5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.1,0.9175,0.9169,0.855 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.607, 66.607, 123.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 1.500 |
| R-factor | 0.1325 |
| R-free | 0.16450 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 1.720 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.246 |
| Total number of observations | 384543 * | |
| Number of reflections | 45181 | |
| <I/σ(I)> | 24 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 | 18 * | PROTEIN WAS CRYSTALLIZED FROM 1.6 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE BUFFER PH 5.3, 0.2 M POTASSIUM SODIUM TARTRATE, 1 MM DTT, 0.02 % (W/V) SODIUM AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | potassium/sodium tartrate | 0.2 (M) | |
| 5 | 1 | reservoir | 1,4-dithio-DL-threitol | 0.001 (M) | |
| 6 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |
