1HCV

LLAMA HEAVY CHAIN VARIABLE DOMAIN AGAINST ALPHA SUBUNIT OF HCG (HUMAN CHORIONIC GONADOTROPIN)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1996-02-19
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	29.620, 41.830, 38.520
Unit cell angles	90.00, 95.45, 90.00

Refinement procedure

Resolution	6.000 - 1.850
R-factor	0.171
Rwork	0.171
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	VH OF FAB FRAGMENT OF ANTIBODY AGAINST 2-PHENYL-OXAZOLONE
RMSD bond length	0.009
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	1.850 *
Rmerge	0.089 *	0.191 *
Total number of observations	92439 *
Number of reflections	8077 *
<I/σ(I)>	9.03	3.4
Completeness [%]	99.8 *	100 *
Redundancy	1.6	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	20 *	VAPOR DIFFUSION, PEG 8K 25%, TRIS 0.1M, PH 8.0, vapor diffusion

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	25 (%)
3	1	reservoir	Tris-HCl	0.1 (M)