1HCG

STRUCTURE OF HUMAN DES(1-45) FACTOR XA AT 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	93.660, 93.660, 119.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.168
RMSD bond length	0.020
RMSD bond angle	0.055
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.087 *
Total number of observations	53755 *
Number of reflections	17121 *
Completeness [%]	61.5 *	38 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	0.2 (M)	precipitant
3	1	drop	HEPES	0.1 (M)	precipitant
4	1	drop	sodium potassium phosphate	2.0 (M)	precipitant
5	1	reservoir	precipitant	1.9 (M)