1HBR

R-STATE FORM OF CHICKEN HEMOGLOBIN D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-03
Detector	SDMS
Spacegroup name	P 1 21 1
Unit cell lengths	53.960, 80.510, 82.110
Unit cell angles	90.00, 104.50, 90.00

Refinement procedure

Resolution	10.000 - 2.300
Rwork	0.222
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hho
RMSD bond length	0.008
RMSD bond angle	21.400 *
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.068 *	0.239 *
Number of reflections	29702
<I/σ(I)>	15.5	2.3
Completeness [%]	99.1	97.7
Redundancy	6.8 *	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG3350	20-30 (%)
2	1	1	salt	10-30 (mM)
3	1	2	protein	20 (mg/ml)
4	1	2	Tris-HCl	50 (mM)