1HBA
HIGH-RESOLUTION X-RAY STUDY OF DEOXYHEMOGLOBIN ROTHSCHILD 37BETA TRP-> ARG: A MUTATION THAT CREATES AN INTERSUBUNIT CHLORIDE-BINDING SITE
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.000, 99.300, 66.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.100 |
| R-factor | 0.176 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.025 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.043 * |
| Total number of observations | 101776 * |
| Number of reflections | 33303 * |
| Completeness [%] | 77.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | hemoglobin | 10 (mg/ml) | |
| 2 | 1 | 1 | potassium phosphate | 10 (mM) | |
| 3 | 1 | 1 | potassium chloride | 100 (mM) | |
| 4 | 1 | 1 | sodium dithionite | 4 (mM) | |
| 5 | 1 | 1 | PEG6000 | 10-10.5 (%) |
