1HB6
Structure of bovine Acyl-CoA binding protein in orthorhombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1991-08-15 |
Detector | MULTIWIRE UCSD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.050, 54.780, 65.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.140 - 2.000 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2abd |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | UCSD |
Data scaling software | UCSD |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.150 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.040 | 0.076 |
Number of reflections | 6473 | |
<I/σ(I)> | 32 | 9.3 |
Completeness [%] | 94.9 | 91.2 |
Redundancy | 3.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 15% PEG8000, 5MMCDCL2, 10MM HEPES PH7.5, pH 7.50 |