1HAP

COMPLEX OF HUMAN ALPHA-THROMBIN WITH A 15MER OLIGONUCLEOTIDE GGTTGGTGTGGTTGG (BASED ON X-RAY MODEL OF DNA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	58.280, 77.610, 100.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.161
R-free	0.17000 *
RMSD bond length	0.015 *
RMSD bond angle	0.056 *
Refinement software	NUCLIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.500	2.800 *
Rmerge	0.097 *
Total number of observations	21631 *
Number of reflections	9225
Completeness [%]	68.0 *	38 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
10	1	reservoir	PEG4000	20 (%(w/v))
11	1	reservoir	isopropanol	8 (%(v/v))
2	1	drop	sodium phosphate	0.025 (M)
3	1	drop	HEPES	0.05 (M)
4	1	drop	ammonium sulfate	0.05 (M)
5	1	drop		0.19 (M)
6	1	drop	PEG4000	10 (%(w/v))
7	1	drop	isopropanol	4 (%(v/v))
8	1	reservoir	ammonium sulfate	0.1 (M)
9	1	reservoir	HEPES	0.1 (M)