1HAH

THE ISOMORPHOUS STRUCTURES OF PRETHROMBIN2, HIRUGEN-AND PPACK-THROMBIN: CHANGES ACCOMPANYING ACTIVATION AND EXOSITE BINDING TO THROMBIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	71.750, 72.390, 73.520
Unit cell angles	90.00, 101.26, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.114
Rwork	0.114
RMSD bond length	0.022
RMSD bond angle	0.052 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.068 *
Number of reflections	15157 *
Completeness [%]	83.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.6 (mg/ml)
2	1	1	sodium phosphate	0.075 (M)
3	1	1		0.18 (M)
4	1	1	PEG8000	15 (%)
5	1	2	PEG8000	18 (%)
6	1	2	sodium phosphate	0.1 (M)
7	1	2	sodium azide	2 (mM)
8	1	2		1 (mM)