1HA7
STRUCTURE OF A LIGHT-HARVESTING PHYCOBILIPROTEIN, C-PHYCOCYANIN FROM SPIRULINA PLATENSIS AT 2.2A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.200, 115.400, 183.040 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.23900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cpc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.135 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.445 |
| Total number of observations | 1177344 * | |
| Number of reflections | 223480 | |
| <I/σ(I)> | 13.1 | 2.5 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.8 | 23% (W/V) PEG 6000, 10% (V/V) OF ETHANOL, 75 MM OF PHOSPHATE BUFFER (PH 6.8) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 23 (%(w/v)) | |
| 3 | 1 | reservoir | ethanol | 10 (%(v/v)) | |
| 4 | 1 | reservoir | phosphate | 75 (mM) | pH6.8 |
