1HA0
HEMAGGLUTININ PRECURSOR HA0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 153.000, 153.000, 153.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.900 * |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.097 |
| Number of reflections | 14885 |
| Completeness [%] | 99.8 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%) | |
| 2 | 1 | reservoir | 75 (mM) | ||
| 3 | 1 | reservoir | HEPES | 50 (mM) | |
| 4 | 1 | reservoir | 0.1 (%) |
