1HA0
HEMAGGLUTININ PRECURSOR HA0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Spacegroup name | I 21 3 |
Unit cell lengths | 153.000, 153.000, 153.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 24.900 * |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.097 |
Number of reflections | 14885 |
Completeness [%] | 99.8 |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 15 (%) | |
2 | 1 | reservoir | 75 (mM) | ||
3 | 1 | reservoir | HEPES | 50 (mM) | |
4 | 1 | reservoir | 0.1 (%) |