1H9V

Human Fc-gamma-Receptor IIa (FcgRIIa), monoclinic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2000-03-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	81.310, 50.000, 55.450
Unit cell angles	90.00, 128.30, 90.00

Refinement procedure

Resolution	50.000 * - 3.000
R-factor	0.246 *
R-free	0.32700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fcb
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.111 *	0.242 *
Number of reflections	3417
Completeness [%]	90.5	69 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.2 *	20 *	0.2M NAOAC PH 4.6, 26% PEG 8000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MOPS	2 (mM)
2	1	drop		150 (mM)
3	1	drop	sodium azide	0.02 (%)
4	1	reservoir	sodium acetate/acetic acid	0.2 (M)
5	1	reservoir	PEG8000	26 (%)