1H9S
Molybdate bound complex of Dimop domain of ModE from E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 41 |
Unit cell lengths | 72.340, 73.340, 49.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.820 |
R-factor | 0.188 * |
Rwork | 0.188 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b9n |
RMSD bond length | 0.010 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 1.820 * | |
Rmerge | 0.075 * | 0.381 * |
Total number of observations | 272924 * | |
Number of reflections | 22803 * | |
<I/σ(I)> | 27.8 | |
Completeness [%] | 98.1 * | 84.3 * |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 | 20 * | pH 7.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | reservoir | trisodium citrate | 1.0 (M) | |
5 | 1 | reservoir | HEPES | 100 (mM) | |
6 | 1 | reservoir | ethylene glycol | 10 (%) | or 1.4M ammonium sulfate |
7 | 1 | reservoir | Tris-HCl | 100 (mM) |