1H9I

COMPLEX OF EETI-II MUTANT WITH PORCINE TRYPSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-03-03
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	139.862, 139.862, 33.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.1416
R-free	0.17550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ldt
RMSD bond length	0.007
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.068	0.217
Number of reflections	26024
<I/σ(I)>	17.84	8.89
Completeness [%]	99.1	97.9
Redundancy	6.6	6.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		pH 6.70