1H9B
ACTIVE MUTANT (Q365->C) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 136.700, 136.700, 121.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.000 - 2.400 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PROTEIN ATOMS OF NADP-BOUND COMPLEX IN THE SAME CRYSTAL FORM 1H9A. |
RMSD bond length | 0.006 |
RMSD bond angle | 22.730 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.113 * | 0.418 * |
Total number of observations | 115220 * | |
Number of reflections | 24865 | 3523 * |
<I/σ(I)> | 12.7 | 3.7 |
Completeness [%] | 94.1 | 93 |
Redundancy | 4.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 * | HANGING DROP VAPOUR DIFFUSION, 2+2 MICROLITER DROPS. THE PROTEIN AT 5MG/ML IN 100MM TRIS-HCL AT PH 7.5 WITH 12.5MM NAD+. DROPS EQUILIBRIATED AGAINST 1.7M UNBUFFERED AMMONIUM SULFATE. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | drop | NAD+ | 12.5 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.64 (M) | unbuffered |