1H99

PRD of LicT antiterminator from Bacillus subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Collection date	1999-11-15
Wavelength(s)	0.979, 0.979, 0.886
Spacegroup name	C 2 2 21
Unit cell lengths	78.250, 129.710, 50.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.550
R-factor	0.224
Rwork	0.224
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	18.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.070	0.132
Number of reflections	37674
<I/σ(I)>	15.6	4.4
Completeness [%]	99.2	98.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		200 MM MG(AC)2, 10% PEG8000, pH 5.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		300 (mM)
2	1	drop	dithiothreitol	5 (mM)
3	1	drop	Tris	10 (mM)
4	1	drop	protein	6 (mg/ml)
5	1	reservoir		200 (mM)
6	1	reservoir	cacodylate	100 (mM)
7	1	reservoir	PEG8000	10 (%)