1H99
PRD of LicT antiterminator from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Collection date | 1999-11-15 |
| Wavelength(s) | 0.979, 0.979, 0.886 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.250, 129.710, 50.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.550 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 18.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.070 | 0.132 |
| Number of reflections | 37674 | |
| <I/σ(I)> | 15.6 | 4.4 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 200 MM MG(AC)2, 10% PEG8000, pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 300 (mM) | ||
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | drop | Tris | 10 (mM) | |
| 4 | 1 | drop | protein | 6 (mg/ml) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | cacodylate | 100 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 10 (%) |
