1H97
Trematode hemoglobin from Paramphistomum epiclitum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0, 1.74 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.700, 83.700, 94.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.170 |
| R-factor | 0.121 |
| R-free | 0.17300 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.190 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.051 | 0.343 |
| Number of reflections | 106124 | |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 91.5 | 84.2 * |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 | 4 * | AMMONIUM SULFATE, PH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | potassium ferricyanide | ||
| 3 | 1 | reservoir | ammonium sulfate | 75 (%sat) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) |
