1H96

recombinant mouse L-chain ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-15
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	180.820, 180.820, 180.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 * - 1.600
R-factor	0.16 *
Rwork	0.160
R-free	0.19000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.009
RMSD bond angle	0.022
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.071 *	0.380 *
Total number of observations	158991 *
Number of reflections	32292 *	2042 *
<I/σ(I)>	9.3	1.9
Completeness [%]	95.7	83.8
Redundancy	4.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293 *	HANGING DROP VAPOR DIFFUSION: DROPS MADE UP OF 3MICROL OF PROTEIN (3.5 MG/ML) IN 20 MM TRIS PH 7.4 AND 3 MICROL OF PRECIPITANT SOLUTION COMPOSED OF 0.92 M AMMONIUM SULFATE, 0.4% CDSO4 AND 3 MM NAN3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.5 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.4
3	1	reservoir	ammonium sulfate	0.92 (M)
4	1	reservoir		0.4 (%)
5	1	reservoir		3 (mM)