1H96
recombinant mouse L-chain ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.820, 180.820, 180.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 * - 1.600 |
| R-factor | 0.16 * |
| Rwork | 0.160 |
| R-free | 0.19000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.071 * | 0.380 * |
| Total number of observations | 158991 * | |
| Number of reflections | 32292 * | 2042 * |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 95.7 | 83.8 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 * | HANGING DROP VAPOR DIFFUSION: DROPS MADE UP OF 3MICROL OF PROTEIN (3.5 MG/ML) IN 20 MM TRIS PH 7.4 AND 3 MICROL OF PRECIPITANT SOLUTION COMPOSED OF 0.92 M AMMONIUM SULFATE, 0.4% CDSO4 AND 3 MM NAN3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 3 | 1 | reservoir | ammonium sulfate | 0.92 (M) | |
| 4 | 1 | reservoir | 0.4 (%) | ||
| 5 | 1 | reservoir | 3 (mM) |
